Reporter

Part:BBa_K2249001

Designed by: Maxwell Saal   Group: iGEM17_CU-Boulder   (2017-10-27)


(EutC1-19)-FusionRed-Luciferase-HA-LVA

BBa_K2249000 with a luciferase fusion, with an HA tag added for purification purposes. We did not end up using this for data collection, but hypothetically it would have been used for an assay where the isolated compartments would have opened up in media containing luciferin, to see if the compartments functioned as an isolator and could be opened with light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 583
    Illegal BsaI.rc site found at 682
    Illegal SapI.rc site found at 1561


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Categories
Parameters
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